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2011•Guide of China MedicineRequires access

Determination of Emodin,Rhein,Chrysophanol,Rheochrysidin and Aloe-emodin in Tongfu Capsule by HPLC

Fan Lei-lei

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Abstract

Objective To develop an HPLC method for determination of Emodin,Rhein,Chrysophanol,Rheochrysidin and Aloe-emodin in Tongfu capsule.MethodsThe analysis was carried out on a Waters C18(250?.6mm,5m) column eluted with a mobile phase of water containing 0.1% phosphoric acid solution-methanol(V∶V=15∶85).The flow rate was 1.0μl/min and column temperature was 35℃.The detection wavelength was set at 254nm.ResultsThe calibration curve was linear within the range of 0.0162~0.323g,0.016~0.318g,0.0164-0.328g,0.008~0.16g,0.020~0.406g for Emodin,Rhein,Chrysophanol,Rheochrysidin and Aloe-emodin,respectively;correlation coefficient were 0.9999.The average recoveries were 99.0%,99.0%,99.2%,98.3%,99.4%(n=6).ConclusionThis method is accurate and reproducible for determination of Emodin,Rhein,Chrysophanol,Rheochrysidin and Aloe-emodin in Tongfu Capsule.

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What this paper is about

Objective To develop an HPLC method for determination of Emodin,Rhein,Chrysophanol,Rheochrysidin and Aloe-emodin in Tongfu capsule.MethodsThe analysis was carried out on a Waters C18(250?.6mm,5m) column eluted with a mobile phase of water containing 0.1% phosphoric acid solution-methanol(V∶V=15∶85).The flow rate was 1.0μl/min and column temperature was 35℃.The detection wavelength was set at 254nm.ResultsThe calibration curve was linear within the range of 0.0162~0.323g,0.016~0.318g,0.0164-0.328g,0.008~0.16g,0.020~0.406g for Emodin,Rhein,Chrysophanol,Rheochrysidin and Aloe-emodin,respectively;correlation coefficient were 0.9999.The average recoveries were 99.0%,99.0%,99.2%,98.3%,99.4%(n=6).ConclusionThis method is accurate and reproducible for determination of Emodin,Rhein,Chrysophanol,Rheochrysidin and Aloe-emodin in Tongfu Capsule.

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Available abstract

Objective To develop an HPLC method for determination of Emodin,Rhein,Chrysophanol,Rheochrysidin and Aloe-emodin in Tongfu capsule.MethodsThe analysis was carried out on a Waters C18(250?.6mm,5m) column eluted with a mobile phase of water containing 0.1% phosphoric acid solution-methanol(V∶V=15∶85).The flow rate was 1.0μl/min and column temperature was 35℃.The detection wavelength was set at 254nm.ResultsThe calibration curve was linear within the range of 0.0162~0.323g,0.016~0.318g,0.0164-0.328g,0.008~0.16g,0.020~0.406g for Emodin,Rhein,Chrysophanol,Rheochrysidin and Aloe-emodin,respectively;correlation coefficient were 0.9999.The average recoveries were 99.0%,99.0%,99.2%,98.3%,99.4%(n=6).ConclusionThis method is accurate and reproducible for determination of Emodin,Rhein,Chrysophanol,Rheochrysidin and Aloe-emodin in Tongfu Capsule.

Key concepts: Emodin, Capsule, Phosphoric acid, Chromatography, High-performance liquid chromatography, Medicine, Aloe emodin, Calibration curve

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Determination of Emodin,Rhein,Chrysophanol,Rheochrysidin and Aloe-emodin in Tongfu Capsule by HPLC — Research Paper | ScholarLens