Simultaneous determination of emodin,chrysophanol,rhein,physcion and aloe-emodin in Maren Wan by HPLC

Objective To establish an HPLC method for the simultaneous determination of emodin,chrysophanol,rhein,physcion and aloe-emodin in Maren Wan.Methods ALLTIMA-C18(4.6 mm×150 mm,5 μm) was used with methanol(A)-0.1% phosphoric acid solution(B) as the mobile phase in gradient elution[0-9 min,60%A;9-20 min,60%-80%A;20-45 min,80%A].The flow rate was 1.0 mL·min-1,and the column temperature was 30℃.The detection wavelength was 254 nm.Results The linear range of emodin,rhein,chrysophanol,phy scion,and aloe-emodin was 9.08-81.72,8.4-75.6,13.42-120.7,7.56-68.04,and 8.2-73.8 μg.mL-1,the average recovery was 99.1 %,99.6 %,99.4 %,99.8 %,and 99.2 %,with RSD 2.00 %,1.75 %,3.20 %,1.03 %,and 1.45 %.Conclusion The method is accurate,sensitive and feasible with good reproductivity,which can be used for the quality control of emodin,chrysophanol,rhein,physcion and aloe-emodinin Maren Wan.