Optimization of Processing Parameters for Extraction of Soluble Polysaccharide of Grifola frondosa

The aim is to optimize the extraction technology of polysaccharides from Grifola frondosa.With the water as the extraction solvent,the effects of particle size,extraction temperature,extraction time,solid-liquid ratio,degree of alcohol precipitation on the yield of polysaccharides from Grifola frondosa are investigated by the single factor experiments and the extraction technology is optimized by using the orthogonal experiment.The results show that the effect of solid liquid radio extraction temperature on the yield of polysaccharides from Grifola frondosa is the largest,the second is the effect of extraction time and the extraction temperature is the least.The optimization technology suitable for local conditions which is selected through optimizing extraction condition and combining the comprehensive factors of benefit,cost and so on is as follows: the extraction temperature is 90 ℃,the extraction time is 5 h and the liquid solid radio is 25∶1.The verification test shows that the yield of polysaccharides extracted under the optimum technology condition is 13.4%.