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Determination of Paeoniflorin in Ruheneixiao Liquid by HPLC

Weidong Wu

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Abstract

Objective To establish a method for the content determination of paeoniflorin in Ruhe Neixiao liquid.Methods The HPLC was used.HYPERSIL C18 column was used(10 μm,4.6 mm×150 mm).The mobile phase was consisted of methanol -0.03 mol/L potassium dihydrogen phosphate(28:72) solution.The flow rate was 1.0 ml/min,and the detection wave length was 230 nm at room temperature.Results The linearity range of paeoniflorin was 0.229 6～1.148 0 μg,r=0.999 6.The precision of the method is reliable.RSD was 1.03%.Sample was stable within 8 h.The average recovery of paeoniflorin was 96.7%,and RSD was 1.33%(n=5).Conclusion The established method is accurate and reproducible.It can be used for the determination of paeoniflorin in Ruhe Neixiao liquid.

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What this paper is about

Objective To establish a method for the content determination of paeoniflorin in Ruhe Neixiao liquid.Methods The HPLC was used.HYPERSIL C18 column was used(10 μm,4.6 mm×150 mm).The mobile phase was consisted of methanol -0.03 mol/L potassium dihydrogen phosphate(28:72) solution.The flow rate was 1.0 ml/min,and the detection wave length was 230 nm at room temperature.Results The linearity range of paeoniflorin was 0.229 6～1.148 0 μg,r=0.999 6.The precision of the method is reliable.RSD was 1.03%.Sample was stable within 8 h.The average recovery of paeoniflorin was 96.7%,and RSD was 1.33%(n=5).Conclusion The established method is accurate and reproducible.It can be used for the determination of paeoniflorin in Ruhe Neixiao liquid.

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Available abstract

Objective To establish a method for the content determination of paeoniflorin in Ruhe Neixiao liquid.Methods The HPLC was used.HYPERSIL C18 column was used(10 μm,4.6 mm×150 mm).The mobile phase was consisted of methanol -0.03 mol/L potassium dihydrogen phosphate(28:72) solution.The flow rate was 1.0 ml/min,and the detection wave length was 230 nm at room temperature.Results The linearity range of paeoniflorin was 0.229 6～1.148 0 μg,r=0.999 6.The precision of the method is reliable.RSD was 1.03%.Sample was stable within 8 h.The average recovery of paeoniflorin was 96.7%,and RSD was 1.33%(n=5).Conclusion The established method is accurate and reproducible.It can be used for the determination of paeoniflorin in Ruhe Neixiao liquid.

Key concepts: Paeoniflorin, Chromatography, Chemistry, High-performance liquid chromatography, Potassium, Phosphate buffered saline, Content determination, Analytical Chemistry (journal)

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