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2009•Anhui Medical and Pharmaceutical JournalRequires access

Determination of baicalin in zhenhuangwan by RP-HPLC

Weidong Chen

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Abstract

Aim To establish a method for the determination of baicalin in zhenhuangwan by HPLC.Method The separation was performed on SinochromODS C18 column,using methanol-water-phosphoric acid(55∶45∶0.2)as mobile phase.The detection wavelength was 280 nm.The flow rate was 1.0 ml·min-1.Result The linear range of baicalin was from 7.824 to 39.12 μg(r=0.999 9).The average recovery was 99.46%,and RSD was 1.3%(n=5).Conclusion The method is simple and accurate.It can be used for the quality control of Zhenhuangwan.

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What this paper is about

Aim To establish a method for the determination of baicalin in zhenhuangwan by HPLC.Method The separation was performed on SinochromODS C18 column,using methanol-water-phosphoric acid(55∶45∶0.2)as mobile phase.The detection wavelength was 280 nm.The flow rate was 1.0 ml·min-1.Result The linear range of baicalin was from 7.824 to 39.12 μg(r=0.999 9).The average recovery was 99.46%,and RSD was 1.3%(n=5).Conclusion The method is simple and accurate.It can be used for the quality control of Zhenhuangwan.

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Available abstract

Aim To establish a method for the determination of baicalin in zhenhuangwan by HPLC.Method The separation was performed on SinochromODS C18 column,using methanol-water-phosphoric acid(55∶45∶0.2)as mobile phase.The detection wavelength was 280 nm.The flow rate was 1.0 ml·min-1.Result The linear range of baicalin was from 7.824 to 39.12 μg(r=0.999 9).The average recovery was 99.46%,and RSD was 1.3%(n=5).Conclusion The method is simple and accurate.It can be used for the quality control of Zhenhuangwan.

Key concepts: Baicalin, Chromatography, Phosphoric acid, High-performance liquid chromatography, Linear range, Chemistry, Recovery rate, Linear relationship

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