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Hindlimb ischemia model in diabetic rabbits

Han Guang-yu

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Abstract

Objective To establish an experimental animal model of limb ischemia in diabetic rabbits.Methods The diabetic model was established by administration of alloxan in 30 rabbits.Ischemia was induced by ligation of the distal iliac artery and excision of the common and superficial femoral arteries in the right hindlimb of 26 rabbits with stable diabetes.All the rabbits were given normal feeding.The severity of the ischemia in each animal was evaluated regularly until 8 weeks after the surgery.Results A total of 30 rabbits were modeled,4 of them died after administration of ALX,26 of them were modeled successfully.The postoperative angiography showed that the count rate of subtle collateral vessels increased from 0.56(s=0.21) to 1.54(s=0.26).The histological study of the hindlimb muscle showed atrophy,fibrosis and necrosis.Conclusions Persistent hindlimb ischemia model in the diabetic rabbit is established successfully,and the model is stable and repeatable.

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What this paper is about

Objective To establish an experimental animal model of limb ischemia in diabetic rabbits.Methods The diabetic model was established by administration of alloxan in 30 rabbits.Ischemia was induced by ligation of the distal iliac artery and excision of the common and superficial femoral arteries in the right hindlimb of 26 rabbits with stable diabetes.All the rabbits were given normal feeding.The severity of the ischemia in each animal was evaluated regularly until 8 weeks after the surgery.Results A total of 30 rabbits were modeled,4 of them died after administration of ALX,26 of them were modeled successfully.The postoperative angiography showed that the count rate of subtle collateral vessels increased from 0.56(s=0.21) to 1.54(s=0.26).The histological study of the hindlimb muscle showed atrophy,fibrosis and necrosis.Conclusions Persistent hindlimb ischemia model in the diabetic rabbit is established successfully,and the model is stable and repeatable.

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Available abstract

Objective To establish an experimental animal model of limb ischemia in diabetic rabbits.Methods The diabetic model was established by administration of alloxan in 30 rabbits.Ischemia was induced by ligation of the distal iliac artery and excision of the common and superficial femoral arteries in the right hindlimb of 26 rabbits with stable diabetes.All the rabbits were given normal feeding.The severity of the ischemia in each animal was evaluated regularly until 8 weeks after the surgery.Results A total of 30 rabbits were modeled,4 of them died after administration of ALX,26 of them were modeled successfully.The postoperative angiography showed that the count rate of subtle collateral vessels increased from 0.56(s=0.21) to 1.54(s=0.26).The histological study of the hindlimb muscle showed atrophy,fibrosis and necrosis.Conclusions Persistent hindlimb ischemia model in the diabetic rabbit is established successfully,and the model is stable and repeatable.

Key concepts: Medicine, Hindlimb, Ischemia, Ligation, Diabetes mellitus, Femoral artery, Atrophy, Animal model

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