Experimental Study on a Hindlimb Ischemia Model in Diabetic Rabbits

Objective To explore and establish the model method of simple,stable,good repeatability about the diabetes rabbit's hind limbs ischemia.Methods Forty rabbits were randomly divided into experimental group(n=30) and control group(n=10).The experimental group were injected twice with alloxan(alloxan,ALX),the control group were injected with normal saline,to establish the model of diabetes rabbit.It was leaded to ischemia due to ligature and dividing the right hind limb's distal iliac artery and resection the superficial brachial artery of the diabetes rabbit's right hind limb.The degree of ischemia was assessed regularly food until 60 days of postoperative.Results In experimental group,30 rabbits were involved in modeling,two rabbits died after the 1st injection of ALX,three died after the 2nd injection of ALX,the rest of 25 rabbits were successful for making the diabetes model.Angiography showed that femoral wispy collateral vessels numeration rate increased from 0.54±0.26 to 1.57±0.28.Histologic appraisal showed there was partial atrophy and necrosis in rabbits' right hind limb muscle.Conclusion It was successful to make the model of stable and good repeatability about the diabetes rabbit's hind limbs ischemia,which was to provide the ideal model for the experimental study of Cell transplantation in the treatment of diabetes.