2001•Zhongguo yaofangRequires access

Determination of Puerarin in Jiangtang Pill by TLC

Dihui Li

Abstract

OBJECTIVE:To develop a method of thin-layer chromatographic scanning to determine the content of puerarin in Jiangtang pill METHODS:Silica GF254 thin-layer was used,and the mobile phase consisted of chloroform-methanol-water(7∶2 5∶0 25) with the detection wave-length of 254nm and the control wave-length of 370nm RESULTS:The linear range of puerarin was 0 4～2 0μg The mean recovery was 99 40%(n=5) with RSD of 1 37% CONCLUSION:This method is accurate,reliable and can be used for the determination of puerarin in Jiangtang pill

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Key concepts: Puerarin, Chromatography, Thin-layer chromatography, Pill, Chemistry, Chloroform, Silica gel, Thin layer