Extraction,Separation and Structural Identification of Chlorogenic Acid from Eucommia ulmoides Leaves

Study the extraction and separation technology of chlorogenic acid from Eucommia ulmoides leaves.Water extracting technology of chlorogenic acid was studied through the orthogonal test.Purification process of chlorogenic acid by column chromatography was discussed.The optimum technological parameters of water extraction were as followed:ratio of leaves to solvent was 1∶8,extracting temperature was 80 ℃,extracting times was twice,the leaves were extracted by hot water for 70 min.With this method,the extraction yield of chlorogenic acid could reach 1.78 %.Chlorogenic acid could be purified by D-101 macroporous resin column chromatography,silica gel column chromatography and Sephadex LH-20 column chromatography.The content of chlorogenic acid in the leaves of leaf-oriented tree model was higher.