Differentiation Induction of Kenaf Adventitious Buds through Two Approaches

Cotyledon and hypocotyl were used as explants to study the influential factors of kenaf adventitious buds differentiation through two approaches.The results showed that:(1) in the approach of callus induction,the quality of hypocotyl callus was better than cotyledon,callus could be induced to differentiate a few adventitious buds in the medium of MS + 6-BA (1mg/L) + 2,4-D (1mg/L) +GA3 (1mg/L),but the induction rate was very low.After adding 0.3% activated carbon in the medium,many roots were induced along with adventitious buds.(2) In the approach of inducing explants directly,hypocotyl hardly generated adventitious buds,cotyledon could induce adventitious effectively.Cotyledon could get the highest induction rate in the medium of MS + TDZ (0.1mg/L) + NAA (0.1mg/L),and the rate was 64.29%.After adding AgNO3 in the medium,the rate could increase obviously.The best induction medium was MS + TDZ (0.1mg/L) + NAA (0.1mg/L) + AgNO3 (1mg/L),and the adventitious buds induction rate was 85%.