Study on Tissue Culture and Rapid Propagation in Vitro of Limonium bicolor

[Objective]The study aimed to provide the scientific direction of rapid propagation technique.[Method] Taking leaf and hypocotyl of Limonium bicolor as the explant,the system of rapid propagation were established,the influences of hormone concentration to induce the tissue culture and adventitious buds were studied.[Result] Different concentration culture medium can induce the explants grew out tissue culture and adventitious buds,A,B,H,I groups of rooting medium can induce adventitious buds generated adventitious roots,the other groups had no adventitious roots.[Conclusion] Taking cotyledons as explant,the best induction medium of tissue culture and adventitious buds were MS0+0.7 mg/L 6-BA+0.1 mg/L NAA,MS0+0.5 mg/L 6-BA+0.1 mg/L NAA respectively;with hypocotyl as explant,the best induction medium of tissue culture and adventitious buds was MS0+0.3 mg/L 6-BA+0.5 mg/L NAA.The best medium for inducing adventitious roots was MS0.