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METHOD FOR RAPID PROPAGATION OF PLANTLETS IN VITRO

Ding Cheng-fang

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Abstract

The traditional method for propagation of plantlets in vitro is to culture the stem with one node in a medium. In this research, the stems of plantlets in vitro of the varieties Atlantic and Wuhua, with multi-node but their top and basal parts removed, were eultured in media with different hormones and different combinations. The MS medium supplemented with 1.0 mg/L BA +1.0 mg/L NAA was in favor of sprouting and rooting of the stem. The plantlets in vitro produced can be subcultured using the same procedure. This method for propagation of plantlets in vitro can reduce the production cost and increase the efficiency.

About this research paper

What this paper is about

The traditional method for propagation of plantlets in vitro is to culture the stem with one node in a medium. In this research, the stems of plantlets in vitro of the varieties Atlantic and Wuhua, with multi-node but their top and basal parts removed, were eultured in media with different hormones and different combinations. The MS medium supplemented with 1.0 mg/L BA +1.0 mg/L NAA was in favor of sprouting and rooting of the stem. The plantlets in vitro produced can be subcultured using the same procedure. This method for propagation of plantlets in vitro can reduce the production cost and increase the efficiency.

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Available abstract

The traditional method for propagation of plantlets in vitro is to culture the stem with one node in a medium. In this research, the stems of plantlets in vitro of the varieties Atlantic and Wuhua, with multi-node but their top and basal parts removed, were eultured in media with different hormones and different combinations. The MS medium supplemented with 1.0 mg/L BA +1.0 mg/L NAA was in favor of sprouting and rooting of the stem. The plantlets in vitro produced can be subcultured using the same procedure. This method for propagation of plantlets in vitro can reduce the production cost and increase the efficiency.

Key concepts: Sprouting, In vitro, Biology, Tissue culture, Botany, Horticulture, Murashige and Skoog medium, Biochemistry

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