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2007•Acta Agriculturae Boreali-SinicaRequires access

Study on Rapid-Multiplication in Vitro and Plantlet Regeneration of Sugerbeets

LI Shu-sheng

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Abstract

Four kinds of explants including seeds,stem segments of seedling,shoot tips with petioles, leaves and axillary bud of eight sweet beets strains were used to study the rapid-multiplication,the regeneration of plantlets,plat transplanting of plantlets in vitro.The results showed:MS+6-BA 0.8～1.5mg/L+ NAA 0.2～0.3mg/L is the best medium for regenerated plantlets induction to all kinds of explants,and the plantlet grows robust.Using MS+NAA 1.0～1.5mg/L or MS+NAA 1.0～1.2mg/L+IBA 0.2mg/L as rooting culture media,the rooting rate will be more than 85%,and root is developed,the survival rate of regenerated plantlets reaches more than 90%.A series of plantlet regeneration and rapid-multiplication in vitro system of sweet beet has been set up,which can also be used as receptor system for gene transfer.

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What this paper is about

Four kinds of explants including seeds,stem segments of seedling,shoot tips with petioles, leaves and axillary bud of eight sweet beets strains were used to study the rapid-multiplication,the regeneration of plantlets,plat transplanting of plantlets in vitro.The results showed:MS+6-BA 0.8～1.5mg/L+ NAA 0.2～0.3mg/L is the best medium for regenerated plantlets induction to all kinds of explants,and the plantlet grows robust.Using MS+NAA 1.0～1.5mg/L or MS+NAA 1.0～1.2mg/L+IBA 0.2mg/L as rooting culture media,the rooting rate will be more than 85%,and root is developed,the survival rate of regenerated plantlets reaches more than 90%.A series of plantlet regeneration and rapid-multiplication in vitro system of sweet beet has been set up,which can also be used as receptor system for gene transfer.

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Available abstract

Four kinds of explants including seeds,stem segments of seedling,shoot tips with petioles, leaves and axillary bud of eight sweet beets strains were used to study the rapid-multiplication,the regeneration of plantlets,plat transplanting of plantlets in vitro.The results showed:MS+6-BA 0.8～1.5mg/L+ NAA 0.2～0.3mg/L is the best medium for regenerated plantlets induction to all kinds of explants,and the plantlet grows robust.Using MS+NAA 1.0～1.5mg/L or MS+NAA 1.0～1.2mg/L+IBA 0.2mg/L as rooting culture media,the rooting rate will be more than 85%,and root is developed,the survival rate of regenerated plantlets reaches more than 90%.A series of plantlet regeneration and rapid-multiplication in vitro system of sweet beet has been set up,which can also be used as receptor system for gene transfer.

Key concepts: Plantlet, Transplanting, Explant culture, Biology, Axillary bud, Shoot, Seedling, Botany

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