Real-time fluorescence quantitative PCR detection of Staphylococcus aureus in experimental study

Objective To establish real-time fluorescent quantitative PCR method for rapid detecting Staphylococcus aureus and investigate its feasibility and application value.Methods Primers and probes were designed based on femB gene sequence of Staphylococcus aureus.Quantitative standard for Staphylococcus aureus was upbuilt by cloning of femB gene.Then real-time fluorescence quantitative PCR for Staphylococcus aureus was established routinely.Results It was identified that femB gene fragment was cloned as standard successfully.Real-time fluorescence quantitative PCR method showed good specificity,sensitivity,stability by the verification experiment.And the coincidence rate was 100%comparing simulation specimens with culture strains.Conclusion The establishment of real-time fluorescent quantitative PCR detection of Staphylococcus aureus provide a basis for rapid detecting food-borne contamination of Staphylococcus aureus.It could be applied to food hygiene regulation,quarantine and clinical diagnosis.