Multiplex fluorescent real-time PCR for detection of methicillin-resistance gene and enterotoxin gene A in Staphylococcus aureus

Objective To establish a multiplex fluorescent real-time PCR assay for the detection of methicillin-resistance gene and enterotoxin gene A of Staphylococcus aureus.Methods We designed the specific primers and TaqMan probes targeting sea,mecA and nuc gene of Staphylococcus aureus.We used different fluorescents to label the probes.After optimization of reaction condition,we set up a multiplex fluorescent real-time PCR assay to detect Staphylococcus aureus rapidly and accurately.Results The result demonstrated that this assay was specific,which can identify three kinds of genes from Staphylococcus aureus in one reaction.The lowest detection limit was 2×10-7μg/μl.Conclusion This assay can be used in food sample detection and rapid identification of Staphylococcus aureus.