Ertility of Different Tomato to Leaves Tissue Culture Differences

The male sterile varieties of tomato and Jidong 216 were used as materials in this paper,took leaves as explants,with different hormones,different concentrations of formulations,induced by different fertility of tomato leaves Callus,adventitious buds and rooting,selected medium organizations of efficient and renewable tomato.The results showed that:MS+6-BA 3.0 mg/L+IAA0.3 mg/L+sucrose 30 g/L+agar 7.5 g/L(pH 5.86) expreesed the best results of the callus induction on Jidong 216,the induction rate was 81.0%.MS+6-BA 2.0 mg/L+IAA 0.3 mg/L+sucrose 30 g/L+agar 7.5 g/L(pH 5.86) expreesed the best results of the leaves on the male sterile varieties,the rate was 66.7%.the stem and bud on Jidong 216 was expressed well induced by MS+6-BA 2.0 mg/L+IAA 0.3 mg/L+sucrose 40 g/L+agar 7.5 g/L,the rate was 64.3%.the adventitious buds on the male sterile varieties was expressed well by MS+6-BA 2.0 mg/L+IAA 0.2 mg/L+sucrose 40 g/L+agar 7.5 g/L,the rate was 58.3%.MS+6-BA 3.5 mg/L+IAA 0.3 mg/L+sucrose 40 g/L+agar 7.5 g/L expreesed the best results of the roots on Jidong 216.