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Callus Induction and Embryogenesis of Ziziphus jujuba

Zhang Cun-zhi

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Abstract

Young embryos of the cultivar 'Longdongmayazao'(Zizypthus jujuba)of 50~60 days after bilossom was induced to ptoduced somatic embryos by three-step.The results showed that the proper medium of producing callus was Nitsch+BA 1.0 mg/L+2,4-D 1.5 mg/L+sucrose 30 g/L+agar 4.0 g/L,the proper medium of producing somatic embryo form callus was MS+BA 1.0 mg/L+IBA 0.2 mg/L+LH 0.5 mg/L+vitamin C 0.1 mg/L+sucrose 30 g/L+LH 0.5 mg/L+vitamin C 0.1 mg/L+sucrose 30 g/L+agar 4.0 g/L.The medium of plantlet formation from somatice embryo was MS+BA 1.0 mg/L+IAA 0.5 mg/L+LH 0.5 mg/L+vitamin C 0.1 mg/L+sucrose 30 g/L+agar 4.0 g/L.

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What this paper is about

Young embryos of the cultivar 'Longdongmayazao'(Zizypthus jujuba)of 50~60 days after bilossom was induced to ptoduced somatic embryos by three-step.The results showed that the proper medium of producing callus was Nitsch+BA 1.0 mg/L+2,4-D 1.5 mg/L+sucrose 30 g/L+agar 4.0 g/L,the proper medium of producing somatic embryo form callus was MS+BA 1.0 mg/L+IBA 0.2 mg/L+LH 0.5 mg/L+vitamin C 0.1 mg/L+sucrose 30 g/L+LH 0.5 mg/L+vitamin C 0.1 mg/L+sucrose 30 g/L+agar 4.0 g/L.The medium of plantlet formation from somatice embryo was MS+BA 1.0 mg/L+IAA 0.5 mg/L+LH 0.5 mg/L+vitamin C 0.1 mg/L+sucrose 30 g/L+agar 4.0 g/L.

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Available abstract

Young embryos of the cultivar 'Longdongmayazao'(Zizypthus jujuba)of 50~60 days after bilossom was induced to ptoduced somatic embryos by three-step.The results showed that the proper medium of producing callus was Nitsch+BA 1.0 mg/L+2,4-D 1.5 mg/L+sucrose 30 g/L+agar 4.0 g/L,the proper medium of producing somatic embryo form callus was MS+BA 1.0 mg/L+IBA 0.2 mg/L+LH 0.5 mg/L+vitamin C 0.1 mg/L+sucrose 30 g/L+LH 0.5 mg/L+vitamin C 0.1 mg/L+sucrose 30 g/L+agar 4.0 g/L.The medium of plantlet formation from somatice embryo was MS+BA 1.0 mg/L+IAA 0.5 mg/L+LH 0.5 mg/L+vitamin C 0.1 mg/L+sucrose 30 g/L+agar 4.0 g/L.

Key concepts: Callus, Sucrose, Agar, Plantlet, Ziziphus jujuba, Botany, Embryo, Murashige and Skoog medium

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