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2006•Zhiwu ziyuan yu huanjingRequires access

Analysis on ISSR marker of primary cultivars of waxberry in Jiangsu and Zhejiang

Jianlin Qian, YU Wen-sheng, Huakun Wang, Xiaoming Lou, Zhen Zhang

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Abstract

Inter-simple sequence repeat(ISSR) was used to analysis genomic DNA variations and genetic relationships of waxberry(Myrica rubra Sieb.et Zucc.) in Jiangsu and Zhejiang provinces.8 of 11 ISSR primers were proved to be polymorphic and informative.Total 58 DNA fragments were amplified and 42 were polymorphic(72.5%).The genetic distance was analysed according ISSR result and a dendrogram was constructed using UPGMA method.All cultivars tested were classified into 5 groups and the excellent individual(Jizaoshu No.1) has a close relationship with 'Xiaoyexidi'.This indicates that there were a wide gene flow and topical isolation between waxberry cultivars of two provinces.Maybe 'Xiaoyexidi' was a parent of 'Jizaoshu No.1'.

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What this paper is about

Inter-simple sequence repeat(ISSR) was used to analysis genomic DNA variations and genetic relationships of waxberry(Myrica rubra Sieb.et Zucc.) in Jiangsu and Zhejiang provinces.8 of 11 ISSR primers were proved to be polymorphic and informative.Total 58 DNA fragments were amplified and 42 were polymorphic(72.5%).The genetic distance was analysed according ISSR result and a dendrogram was constructed using UPGMA method.All cultivars tested were classified into 5 groups and the excellent individual(Jizaoshu No.1) has a close relationship with 'Xiaoyexidi'.This indicates that there were a wide gene flow and topical isolation between waxberry cultivars of two provinces.Maybe 'Xiaoyexidi' was a parent of 'Jizaoshu No.1'.

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Available abstract

Inter-simple sequence repeat(ISSR) was used to analysis genomic DNA variations and genetic relationships of waxberry(Myrica rubra Sieb.et Zucc.) in Jiangsu and Zhejiang provinces.8 of 11 ISSR primers were proved to be polymorphic and informative.Total 58 DNA fragments were amplified and 42 were polymorphic(72.5%).The genetic distance was analysed according ISSR result and a dendrogram was constructed using UPGMA method.All cultivars tested were classified into 5 groups and the excellent individual(Jizaoshu No.1) has a close relationship with 'Xiaoyexidi'.This indicates that there were a wide gene flow and topical isolation between waxberry cultivars of two provinces.Maybe 'Xiaoyexidi' was a parent of 'Jizaoshu No.1'.

Key concepts: UPGMA, Dendrogram, Myrica rubra, Cultivar, Genetic relationship, Biology, genomic DNA, Genetic distance

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Analysis on ISSR marker of primary cultivars of waxberry in Jiangsu and Zhejiang — Research Paper | ScholarLens