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Analysis on ISSR Markers of Some Cultivars of Waxberry in Jiangsu Province

YU Wen-sheng

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Abstract

[Objective] This study aimed to investigate the genetic relationship among waxberry(Myrica rubra Bieb.et Zucc) cultivars in Jiangsu Province.[Method] Eighteen waxberry cultivars(lines) in Jiangsu Province were analyzed using ISSR-PCR method,and molecular phylogenetic tree was constructed using UPGMA method.[Result] A total of 70 DNA fragments were amplified with nine screened primers,including 43 polymorphic fragments,accounting for 61.4% of the total number of amplified fragments;18 waxberry cultivars(lines) were clustered into 6 categories with the genetic distance of 0.14,indicating that there is partial isolation on the basis of gene exchange between waxberry cultivars(lines) in Jiangsu Province.[Conclusion] This study provided scientific basis for the protection and utilization of waxberry germplasm resources and cultivation of new varieties.

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What this paper is about

[Objective] This study aimed to investigate the genetic relationship among waxberry(Myrica rubra Bieb.et Zucc) cultivars in Jiangsu Province.[Method] Eighteen waxberry cultivars(lines) in Jiangsu Province were analyzed using ISSR-PCR method,and molecular phylogenetic tree was constructed using UPGMA method.[Result] A total of 70 DNA fragments were amplified with nine screened primers,including 43 polymorphic fragments,accounting for 61.4% of the total number of amplified fragments;18 waxberry cultivars(lines) were clustered into 6 categories with the genetic distance of 0.14,indicating that there is partial isolation on the basis of gene exchange between waxberry cultivars(lines) in Jiangsu Province.[Conclusion] This study provided scientific basis for the protection and utilization of waxberry germplasm resources and cultivation of new varieties.

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Available abstract

[Objective] This study aimed to investigate the genetic relationship among waxberry(Myrica rubra Bieb.et Zucc) cultivars in Jiangsu Province.[Method] Eighteen waxberry cultivars(lines) in Jiangsu Province were analyzed using ISSR-PCR method,and molecular phylogenetic tree was constructed using UPGMA method.[Result] A total of 70 DNA fragments were amplified with nine screened primers,including 43 polymorphic fragments,accounting for 61.4% of the total number of amplified fragments;18 waxberry cultivars(lines) were clustered into 6 categories with the genetic distance of 0.14,indicating that there is partial isolation on the basis of gene exchange between waxberry cultivars(lines) in Jiangsu Province.[Conclusion] This study provided scientific basis for the protection and utilization of waxberry germplasm resources and cultivation of new varieties.

Key concepts: UPGMA, Cultivar, Genetic relationship, Germplasm, Myrica rubra, Biology, Phylogenetic tree, Phylogenetic relationship

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Analysis on ISSR Markers of Some Cultivars of Waxberry in Jiangsu Province — Research Paper | ScholarLens