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2005•Strait Pharmaceutical JournalRequires access

Determination of Puerarin in Yuye XiaoKe Granules by HPLC

Wang Li

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Abstract

OBJECTIVE To establish a method for the determination of puerarin in Yuye Xiaoke granules by HPLC.METHODS The HPLC column was Lichrospher-C_ 18 (4.6mm×250mm,5μm);The mobile phase was methanol-0.4% acetic acid (26∶74);The flow rate was 1.0ml·min -1 ;the detection wavelength was 250nm.RESULTS The Calibration curve of puerarin was good Liner relationship in the range of 0.0103μg～0.2060μg,Y=4651796.7x+2955.3,R=0.99999;the average recovery was 102.4%,RSD was 1.1% (n=9).CONCLUSION The method is simple、rapid、accurate、dependable and specific.The method can be used to control the quality of Yuye Xiaoke granules.

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What this paper is about

OBJECTIVE To establish a method for the determination of puerarin in Yuye Xiaoke granules by HPLC.METHODS The HPLC column was Lichrospher-C_ 18 (4.6mm×250mm,5μm);The mobile phase was methanol-0.4% acetic acid (26∶74);The flow rate was 1.0ml·min -1 ;the detection wavelength was 250nm.RESULTS The Calibration curve of puerarin was good Liner relationship in the range of 0.0103μg～0.2060μg,Y=4651796.7x+2955.3,R=0.99999;the average recovery was 102.4%,RSD was 1.1% (n=9).CONCLUSION The method is simple、rapid、accurate、dependable and specific.The method can be used to control the quality of Yuye Xiaoke granules.

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Available abstract

OBJECTIVE To establish a method for the determination of puerarin in Yuye Xiaoke granules by HPLC.METHODS The HPLC column was Lichrospher-C_ 18 (4.6mm×250mm,5μm);The mobile phase was methanol-0.4% acetic acid (26∶74);The flow rate was 1.0ml·min -1 ;the detection wavelength was 250nm.RESULTS The Calibration curve of puerarin was good Liner relationship in the range of 0.0103μg～0.2060μg,Y=4651796.7x+2955.3,R=0.99999;the average recovery was 102.4%,RSD was 1.1% (n=9).CONCLUSION The method is simple、rapid、accurate、dependable and specific.The method can be used to control the quality of Yuye Xiaoke granules.

Key concepts: Puerarin, Chromatography, Calibration curve, High-performance liquid chromatography, Chemistry, Methanol, Acetic acid, Analytical Chemistry (journal)

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