ScholarLens Back to search
2001•Yaowu fenxi zazhiRequires access

RP-HPLC Determination of Puerarin in Ganmao Qingre Granules

Xiaoping Yang, Rao Yan-ping

Open publisher page 0 citations

Abstract

Objective:To establish a HPLC method for determination of the content of puerarin in Ganmao Qing re granules.Methods:The condition of RP-HPLC was Suplcosil C 16 column(150 mm×4\^6 mm,5 μm),mobile phase of methanol-water (27∶73)with a flow rate of 1 0 mL·min -1 and UV detector at 248 nm.Results:The calibration curve was linear ( r =0 999 9)within the range of 0 2～200 μg·mL -1 for puerarin,the average recovery was 101 7%( n =3).Conclusion:This method is simple,rapid and reliable,suitable for the quality control of the drug.

About this research paper

What this paper is about

Objective:To establish a HPLC method for determination of the content of puerarin in Ganmao Qing re granules.Methods:The condition of RP-HPLC was Suplcosil C 16 column(150 mm×4\^6 mm,5 μm),mobile phase of methanol-water (27∶73)with a flow rate of 1 0 mL·min -1 and UV detector at 248 nm.Results:The calibration curve was linear ( r =0 999 9)within the range of 0 2～200 μg·mL -1 for puerarin,the average recovery was 101 7%( n =3).Conclusion:This method is simple,rapid and reliable,suitable for the quality control of the drug.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Objective:To establish a HPLC method for determination of the content of puerarin in Ganmao Qing re granules.Methods:The condition of RP-HPLC was Suplcosil C 16 column(150 mm×4\^6 mm,5 μm),mobile phase of methanol-water (27∶73)with a flow rate of 1 0 mL·min -1 and UV detector at 248 nm.Results:The calibration curve was linear ( r =0 999 9)within the range of 0 2～200 μg·mL -1 for puerarin,the average recovery was 101 7%( n =3).Conclusion:This method is simple,rapid and reliable,suitable for the quality control of the drug.

Key concepts: Puerarin, Chemistry, Chromatography, High-performance liquid chromatography, Calibration curve, Linear range, Methanol, Detection limit

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
RP-HPLC Determination of Puerarin in Ganmao Qingre Granules — Research Paper | ScholarLens