Optimization of Extraction Collagen from Scales Using Acid and Enzymatic Composite Process

Fish scales were used as raw material to extract collagen with acid and enzymatic composite process after pretreatment. The optimal solvent for removing impurity proteins and mineral impurities from fish scales respectively with 0.1 mol / L Na Cl and 0.3 mol / L EDTA. The main factors affecting the efficiency of the extraction of collagen were respectively : citric acid concentration, enzyme dosage and Time. To ensure the invariance of protein, the optimal parameters for extraction were3% citrate acid,amount of enzyme on the basis of 7% the weight of fish scales for 6 h at 25 ℃(room temperature). Under these conditions,the extraction amount of hydroxyproline was up to 2.395 μg / g. Extraction collagen from scales using acid and enzymatic composite process,which not only ensure the integrity of collagen, but also helps to improve the yield.