Determination of oleanolic acid and ursolic acid content in six cultivars of Zizyphus Jujuba by RP-HPLC

To establish a method for the content determination of oleanolic acid and ursolic acid in Zizyphus Jujuba of Xinjiang.The content of oleanolic acid and ursolic acid was detected by RP-HPLC.The separation was carried out on a Kromasil C18 column(250 mm×4.6 mm,5 μm).The mobile phase was methanol-0.03 mol/L Sodium Dihydrogen Phosphate(adjusted to pH 3.0 with phosphoric acid)(90: 10);the flow rate was 0.8 mL/min;the detective wavelength was set at 210 nm.The column temperature was 25 ℃.The results indicated two compounds were basically isolated.The linear ranges of oleanolic acid and ursolic acid were 0.4~2.0 μg(r=0.999 8) and 0.448~2.24 μg(r=0.999 9),respectively.The average recoveries were 99.4% with RSD of 1.33% and 99.6% with RSD of 1.43 %(n=6),respectively.This method was rapid,accurate,reliable;it could be used as a determination of oleanolic acid and ursolic acid content method for Zizyphus Jujuba.