Effect of Euryale Ferox on Expression of Urotensin II and Collagen I,III in Renal Tissues of Diabetic Nephropathy Rats

Objective: To observe the effect of euryale ferox on expression of Urotensin Ⅱ and collagen in diabetic nephropathy rats. Methods: Sixty male Sprague- Dawley( SD) rats weighing 200 ~ 220 g were used. Randomly selected 10 rats in control group( group N),the remaining 50 making DN models injected streptozotocin into abdominal cavity( STZ,45 mg /kg),were randomly divided into DN group( group DN),low- dose Euryale ferox group( group EL,1. 5 g·kg- 1·d- 1),medium-dose Euryale ferox group( group EM,3. 0 g·kg- 1·d- 1),high-dose Euryale ferox group( group EH,6.0 g·kg- 1·d- 1) and losartan potassium group( group LP,30 mg·kg- 1·d- 1),10 rats in each group. The rats of group EL,EM,EH and group LP were administrated by gavage. Rats of the other two groups were offered distilled water as much. Each group rats were administered continuously after12 weeks,records of biochemical indicators and observes the renal pathological changes through HE and Masson dyeing. the immunohistochemical and western blot methods were used to observe the expression of UrotensinII,collagen Ⅰ,Ⅲ in renal tissues. Results:( 1) As compared with Control group,DN group rats at the end of the12 week,the glomerular of the rats became hypertrophic and mesangial matrix increased,interstitial collagen deposition,24h urinary protein,serum BUN,Scr levels were significantly higher( P 0. 05),U Ⅱ,collagen Ⅰ,Ⅲ expression increased( P 0. 05).( 2) Compared with group DN,the pathology changes were relieved,BUN,Scr,24 h urinary protein as well as the expression of U Ⅱ,collagen Ⅰ,Ⅲ decreased in group EM and group EH( P 0.05). Conclusion: Euryale ferox may inhibit overexpression of U Ⅱ and the collagen Ⅰ,Ⅲ in renal interstitial,improve kidney function,delay diabetic nephropathy tubulointerstitial fibrosis,and thus play a role in renal protection.