Effect of Euryale Ferox on the Expression of SOCS-3、IGF-1 in Renal Tissues of Diabetic Nephropathy Rats

Objective: To investigate the effect and mechanism of euryale ferox on the expression of SOCS- 3 and IGF- 1in renal tissues of diabetic nephropathy( DN) rats. Methods: The rat models of DN established by a single injection of streptozotocin were randomly divided into 5 groups,namely diabetic nephropathic model group( group DN),low- dose Euryale ferox group( EL,1. 5 g·kg- 1·d- 1),medium- dose Euryale ferox group( EM,3. 0 g·kg- 1·d- 1),high- dose Euryale ferox group( EH,6. 0 g·kg- 1·d- 1) and losartan potassium group( LP,30 mg·kg- 1·d- 1). With normal rats as the contro1,each group of 10.All the rats received daily gavage for 12 weeks,24 h urinary protein( 24 hUpr),blood urea nitrogen( BUN) and blood serum creatinine( Scr) were measured after the research. The renal pathological changes were observed by histochemistry staining and electron microscope. The expression of SOCS- 3 and IGF- 1 was detected by immunohistochemistry and western blot. Results: Compared with group NC,there were the glomerular hypertrophy,proliferation of mesangial cells,the accumulation of mesangial matrix and renal tubular expansion in group DN. 24 h Upr,BUN and Scr also increased significantly( P 0. 05). Meanwhile the expression of SOCS-3 and IGF- 1 in renal tissues increased significantly( P 0. 05). Compared with group DN,the pathology changes were relieved,24 h Upr,BUN,Scr,as well as the expression of IGF- 1 decreased and SOCS- 3 increased discinctly in group EM and group LP( P 0.05). In addition,there was no significant difference in effect of reducing urinary protein in group EM and LP. Conclusion:Euryale ferox may reduce proteinuria and protect renal function by up- regulating the expression of SOCS- 3,inhibiting the overexpression of IGF- 1.