Effect of Euryale Ferox on the Expression of GLUT1 and TGF-β_1 in the Renal Tissues of Diabetic Nephropathy Rats

Objective: To investigate the effect of euryale ferox on the expression of GLUT1 and TGFβ1 in renal tissues of diabetic nephropathy rats. Methods: 60 SD male rats were randomly divided into 6 groups : the normal control group( group N),diabetic nephropathic model group( group DN),low- dose Euryale ferox group( EL,1. 5 g·kg- 1·d- 1),medium- dose Euryale ferox group( EM,3. 0g / kg / d),high- dose Euryale ferox group( EH,6. 0 g·kg- 1·d- 1) and losartan potassium group( LP,30 mg·kg- 1·d- 1). Rats were treated respectively by intragastric administration for 12 weeks. Meanwhile rats of group N and group DN received the same volume distilled water by gavage. Blood serum creatinine,blood urea nitrogen and 24 h urinary protein were measured after the research. The renal pathological changes were observed by histochemistry staining and electron microscope. The expression of GLUT1 and TGF- β1was detected by immunohistochemistry and western blot. Results: Compared with group N,the glomerular became hypertrophic and the mesangial matrix increased in group DN. BUN,Scr,24 h urinary protein also increased significantly( P 0. 05). Meanwhile the expression of GLUT1 and TGF- β1in renal tissues increased significantly( P 0. 05). Compared with group DN,the pathology changes were relieved,BUN,Scr,24 h urinary protein as well as the expression of GLUT1 and TGF- β1decreased in group EM and group EH( P 0. 05). In addition,there was no significant difference in effect of reducing erinary protein in group EM,EH and LP. Conclusion: Euryale ferox may delay the injury in diabetic nephropathy by down- regulating the expression of GLUT1 and TGF- β1.