Effects of Xiezhuo Jiedu Decoction on expression of IL-10, TNF-α and NF-κB in ulcerative colitis rats.

Objective:To observe the effects and mechanism of Xiezhuo Jiedu Decoction on the expression of IL-10,TNF-α and NF-κB in ulcerative colitis rat.Methods:2,4,6-trinitrobenzene sulfonic acid(TNBS)/ethanol solution was administrated by enema to establish ulcerative colitis rats models.Five groups were set up in the experiments:normal control group,model control group,salazosulfamide group,Xiezhuo Jiedu Decoction low-dose group,Xiezhuo Jiedu Decoction high-dose group;Pathomorphological observation was carried out on the rats'colon(DAI scores and CMDI scores).Cytokines IL-10,TNF-α were determined by radioimmunoassay;NF-κB p65 was determined by immunohistochemical staining(SP method).Results:Xiezhuo Jiedu Decoction can significantly reduce the CMDI sores of the rats'colon and the expression of NF-κB p65,increase the content of IL-10,decrease the content of TNF-α.Conclusion:Xiezhuo Jiedu Decoction had good therapeutic effect on ulcerative colitis in rats,its mechanism may be related to reducing the expression of NF-κB p65,increasing the content of IL-10,decreasing the content of TNF-α.