ScholarLens Back to search
2006•Journal of Guangxi Academy of SciencesRequires access

Determination of Oleandic Acid in Herba Isodonis Ternifoliae by HPLC

Yong Chen

Open publisher page 0 citations

Abstract

A HPLC method was developed.The Elite Hypersil ODS2 column was used,and mobile phase was composed of CH_3OH-0.1%H_3PO_4 solution(80∶20),the detection wavelength was at 207 nm and the column temperature was 22℃.The result showed that oleandic acid had the linear range of 0.175~7μg,the average recovery of oleandic acid was 98.91%,RSD=1.886%.The method of quantification is accurate,realiable and reproducible.It can be used effectively for the HPLC determination of oleandic acid in Herba isodonis ternifoliae.

About this research paper

What this paper is about

A HPLC method was developed.The Elite Hypersil ODS2 column was used,and mobile phase was composed of CH_3OH-0.1%H_3PO_4 solution(80∶20),the detection wavelength was at 207 nm and the column temperature was 22℃.The result showed that oleandic acid had the linear range of 0.175~7μg,the average recovery of oleandic acid was 98.91%,RSD=1.886%.The method of quantification is accurate,realiable and reproducible.It can be used effectively for the HPLC determination of oleandic acid in Herba isodonis ternifoliae.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

A HPLC method was developed.The Elite Hypersil ODS2 column was used,and mobile phase was composed of CH_3OH-0.1%H_3PO_4 solution(80∶20),the detection wavelength was at 207 nm and the column temperature was 22℃.The result showed that oleandic acid had the linear range of 0.175~7μg,the average recovery of oleandic acid was 98.91%,RSD=1.886%.The method of quantification is accurate,realiable and reproducible.It can be used effectively for the HPLC determination of oleandic acid in Herba isodonis ternifoliae.

Key concepts: Chromatography, High-performance liquid chromatography, Chemistry, Linear relationship, Linear range, Detection limit, Mathematics, Statistics

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Determination of Oleandic Acid in Herba Isodonis Ternifoliae by HPLC — Research Paper | ScholarLens