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Determination of Apigenin in Herba Spirodrlae by HPLC

Benhua Su

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Abstract

Objective:To determine the Apigenin in Herba Spirodrlae by HPLC.Method:The column employed was Phenomenex-ODS Column(4.6mm×250mm,5μm);the mobile phase was methanol-0.2% phosphoric acid(55:45).The flow rate was 1.0ml/min;detection was affected at 340nm and column temperature was 40 ℃.Result:The linear range of Apigenin was 0.101 68~1.01 68μg(r=0.999 9),and the mean recovery was 98.91%,RSD=1.07%(n=6).Conclusion:This method is simple,rapid and accurate with good reproducibility.It is suitable for the quality control of Herba Spirodrlae.

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What this paper is about

Objective:To determine the Apigenin in Herba Spirodrlae by HPLC.Method:The column employed was Phenomenex-ODS Column(4.6mm×250mm,5μm);the mobile phase was methanol-0.2% phosphoric acid(55:45).The flow rate was 1.0ml/min;detection was affected at 340nm and column temperature was 40 ℃.Result:The linear range of Apigenin was 0.101 68~1.01 68μg(r=0.999 9),and the mean recovery was 98.91%,RSD=1.07%(n=6).Conclusion:This method is simple,rapid and accurate with good reproducibility.It is suitable for the quality control of Herba Spirodrlae.

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Available abstract

Objective:To determine the Apigenin in Herba Spirodrlae by HPLC.Method:The column employed was Phenomenex-ODS Column(4.6mm×250mm,5μm);the mobile phase was methanol-0.2% phosphoric acid(55:45).The flow rate was 1.0ml/min;detection was affected at 340nm and column temperature was 40 ℃.Result:The linear range of Apigenin was 0.101 68~1.01 68μg(r=0.999 9),and the mean recovery was 98.91%,RSD=1.07%(n=6).Conclusion:This method is simple,rapid and accurate with good reproducibility.It is suitable for the quality control of Herba Spirodrlae.

Key concepts: Phosphoric acid, Chromatography, Apigenin, High-performance liquid chromatography, Reproducibility, Chemistry, Linear range, Linear relationship

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