Purification of Flavonoids from Humulus by Macroporous Resin's Absorption

The purpose of this paper is to purify flavonoids from humulus.The experiment determined with absorption and desorption rate as indexes to observe 6 types of macroporous resin's absorption and desorption ability for flavonoids from humulus.Results showed that the HPD-722 macroporous resin has good absorbing and desorbing effect.And the experiment established the optimum process conditions for purification: The liquid concentration of absorbing and separating is 4mg/ml at the injection rate of 2 BV/h,And the sample volume is 5BV.The elution effect of 70% alcohol with 5BV at the speed of 2 BV/h is the best.After purified by HPD-722 macroporous resin,the purification of total flavonoids from persimmon leaves is up to 52%,which is purified by 33.8%.