Simultaneous Determination of 3 Index Components in Buyang Huanwu Decoction by RP-HPLC

OBJECTIVE:To establish the method for simultaneous determination of paeoniflorin,calycosin and ferulic acid in Buyang huanwu decoction.METHODS:RP-HPLC method was adopted.The determination was performed on Odyssil C 18(250 mm× 4.6 mm,5 μm)column with mobile phase consisted of acetonitrile-water-0.1% phosphate acid(16:64:20,V/V/V)at the flow rate of 1.0 ml/min.The detection wavelength was set at 230 nm(paeoniflorin),260 nm(calycosin)and 328 nm(ferulic acid).The column temperature was 30 ℃.RESULTS:The linear ranges were 0.096 4-0.964 0 μg(r=0.999 2)for paeoniflorin,0.099 0-0.990 0 μg for calycosin(r=0.999 6)and 0.008 36-0.083 6 μg for ferulic acid(r=0.999 4),respectively.The average recoveries were 99.72% for paeoniflorin(RSD=0.5%,n=6),99.14% for campanulin(RSD=1.1%,n=6)and 97.96% for ferulic acid(RSD=1.4%,n= 6).CONCLUSION:The method is simple,quick,sensitive and reproducible for the quality control of Buyang huanwu decoction.