2005•Study Journal of Traditional Chinese MedicineRequires access

Determination of Chrysophonol in Huangzhi Oral Liquid by HPLC

Zeng Cong-yan

Abstract

Objective:To establish a HPLC method to determine the content of chrysphonol in Huangzhi oral liquid and control its quanlity.Methods:Under normal condition,using Nova-pak C 18 column(3.9mm×150mm,5μm),mobile phrase was methanol-0.1% phosphoric acid (85:15),with a detection wavelength of 254nm.Results:There was a good linear relationship within the concentration range of 1.696～8.480μg/mL of chrysophonol in Huangzhi oral liquid (r=0.9998).The average recovery and the relative standard deviation of puerarin were 99.49% and 0.62 respectively.Conclusion:The method is simple,accurate and reliable,and can be used for the quality control of Huangzhi oral liquid.

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Key concepts: Chromatography, Phosphoric acid, High-performance liquid chromatography, Linear range, Puerarin, Relative standard deviation, Chemistry, Medicine