Studies on Explants Selection and Callus Induction of Anoectochilus roxburghii

With the stems,leaves,segments,buds of Anoectochilus roxburghiias as explants,the induction of callus was studied by culturing at 5 different culture mediums with 6-BA,ZT,NAA,KT respectively.The results showed that explants including stems,segments and buds could successfully induce callus occurrence at following culture mediums,MS + 6-BA 2.0 mg/L + NAA 0.5 mg/L,MS + NAA 2.0 mg/L + KT 0.1 mg/L and MS + 6-BA 2.0 mg/L + NAA 0.5 mg/L + ZT 0.2 mg/L.Certainly,6-BA,NAA,KT were the important factors on production of callus.Among the three explants,the buds were the best explants for the callus induction.And the results of orthogonal design showed that for the bud of Anoectochilus roxburghii to form the callus,the best medium was MS + 6-BA 2.0 mg/L + NAA 0.5 mg/L + KT 0.2 mg/L.