Effects of Huaiqihuang on IL-5 and INF-γ level of BALF in asthmatic rats

Objective To investigate the effect of Huaiqihuang on IL-5 and INF-γ level of BALF in asthmatic rats. Methods The rats were sensitized and challenged by ova-albumin to establish the asthmatic model.40 rats were randomly divided into 4 groups:normal control group(N),asthmatic model group(A),dexamethasone group(D),dexamethasone + Huaiqihuang group(H).The total cell numbers and eosinophils were counted with a hemocytometer.The level of OVA-IgE in serum and IL-5 and INF-γ in BALF were measured by ELISA. Results The count of EOS in BALF,level of OVA-IgE in serum,IL-5 in BALF:group A was higher than group N(P0.05);group D and group H were lower than group A(P0.05);and group H was lower than group D(P0.05),but mainly the same as group N(P0.05).The level of INF-γ in BALF:group A was lower than group N(P0.05); group D and group H were higher than group A(P0.05);and group H was even higher than group D(P0.05),but mainly the same as group N(P0.05). Conclusion The adjuvant therapy of Huaiqihuang can suppress the high expression of OVA-IgE and IL-5,raise the low expression of INF-γ,regulate Th1/Th2 balance,and it plays an important role in developing airway inflammation.