Experimental Study on Mechanism of Kidney-Tonifying,Spleen-Strengthening and Constitution-Consolidating Method in the Treatment of Bronchial Asthma

In order to explore the functional mechanism of the kidney-tonifying,spleen-strengthening and constitution-consolidating method in the treatment of brachial asthma,72 male SD rats were divided randomly into six groups of the control group(A),asthma model group(B),small dose group of Wheezing-Stopping Capsules(C),large dose group ofWheezing-Stopping Capsules(D),medication control group(E),and TCM and WIM-integrated group(F),12 rats in each group. Beside Group A,egg albumin was injected into the abdominal cavity plus the method to kill Bordetella pertussis,to create asthma model in the rats.Then,the model rats in various groups were given different medicines respectively.Two weeks later,the rats were killed to determine NO,ET-1 and IL-5 contents in plasma and bronchial alveolar lavage fluid(BALF)in the rats of various groups.The results showed that NO concentration and ET-1 content in BALF were obviously higher in Group B than in Group A.In comparison with Group B,the indexes remarkably dropped in Group D and E,but without statistical difference in the latter two groups,NO concentration in BALF was obviously lower in Group F than in Group D and E,closing to Group A.Plasma and IL-Content in BALF were obviously higher in Group B than in Group A.In comparison with Group B,except Group C,these indexes remarkably dropped in the rest groups,in which Group F was obviously lower than in Group D and E,colsing to Group A.The findings indicate that to relieve inflammation in the airway,reconstruct the epithelium of the airway in asthma and decrease high allergy in the airway by reducing plasma and IL-5 in BALF and inhibiting the synthesis and discharge of NO and ET-1 in BALF can be one of the mechanism in the treatment of asthma by the kidney-tonifying,spleen-strengthening and constitution-consolidating method.