The establishment of traditional Chinese medicine animal model of the cold asthma

Objective: To explore the establishment of animal model consistent with the traditional Chinese medicine clinical morbility feature of the cold asthma. Methods: 50 Clean healthy male SD rats were randomly divided into 5 groups: the normal control group (Group A), Western medicine control group (Group B), Traditional Chinese Medicine (TCM) mode 1 group (Group C), TCM mode 2 group (Group D) and TCM mode 3 group (Group E), 10 in each group. Test the changes in cells of rats in BALF, the changes in levels of IL-4, IFN-γ in BALF and also the changes in histopathology of rat lung. Results: ①The total number of cells and percentage of EOS in BALF of Group B, C, D, E were higher than that of normal control group (P0.01), and the total number of cells and percentage of EOS in BALF of Group C rats was higher signifi cantly than that of Group B (P0.01). ②The content of IFN-γ in BALF of Group B, C, D, E rats were lower than that of Group A (P0.01),and Group comparison of Group C and Group B is existence signifi cant difference (P0.05). The contents of IL-4 in BALF of Group B, C, D, E rats were higher than that of Group A (P0.01); the content of IL-4 of TCM Model Group C, Group D, Group E comparison with Group B were significant differences statistically (P0.05), and the differences of Group C were the most obvious in of all (P0.01), Chinese medicine model Group D, Group E comparison with Group C were significantly different (P0.05). ③Rats of model group have shown the pathological changes of asthma inflammatory response. Conclusion: Changes of eosinophil percentage, concentration of IL-4 and INF-γ and pathological in BALF of rat may be the main evidence of evaluation to TCM animal model of the cold asthma.