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Methods of extraction of genomic DNA from wheat

Binbin Si, Chao Zhang, Ruhong Xu

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Abstract

This study aimed at comparing the different methods of DNA extraction from wheat. The results showed that the DNA quality extracted by standard operation was good, but it spent so much time and money. The DNA concentration and purity extracted by improved method were not as good as standard, but it also met the DNA requirements of major molecular research . The improved CTAB extraction method which was designed by our research group is suitable to extract the high quality DNA from wheat. Facile operation can save time and be economical. The quality of DNA extracted by improved CTAB method is so good that it fits for AFLP experiment.

About this research paper

What this paper is about

This study aimed at comparing the different methods of DNA extraction from wheat. The results showed that the DNA quality extracted by standard operation was good, but it spent so much time and money. The DNA concentration and purity extracted by improved method were not as good as standard, but it also met the DNA requirements of major molecular research . The improved CTAB extraction method which was designed by our research group is suitable to extract the high quality DNA from wheat. Facile operation can save time and be economical. The quality of DNA extracted by improved CTAB method is so good that it fits for AFLP experiment.

Why it matters

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Key contribution

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Method / approach

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Main findings

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Available abstract

This study aimed at comparing the different methods of DNA extraction from wheat. The results showed that the DNA quality extracted by standard operation was good, but it spent so much time and money. The DNA concentration and purity extracted by improved method were not as good as standard, but it also met the DNA requirements of major molecular research . The improved CTAB extraction method which was designed by our research group is suitable to extract the high quality DNA from wheat. Facile operation can save time and be economical. The quality of DNA extracted by improved CTAB method is so good that it fits for AFLP experiment.

Key concepts: DNA extraction, DNA, Extraction (chemistry), Amplified fragment length polymorphism, genomic DNA, Chromatography, Chemistry, Computer science

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