Comparison on Several Extraction Methods of Genomic DNA from Hevea brasiliensis

[Objective]Four kinds of extraction methods of genomic DNA from rubber tree were compared to guide people to choose the right DNA extraction method for follow-up experiments for the different needs.[Method]DNA was extracted from rubber tree by TIANGEN DNA secure plant kid,E.Z.N.A.TMHigh Performance(HP) Plant DNA Kit,CTAB method,and improved CTAB method.The purity and concentration of DNA extracted by different methods were determined.And they were detected by electrophoresis and restriction enzyme digestion.[Result]The genomic DNA extracted by improved CTAB method had the highest purity and the best quality.But the DNA extracted by the other three methods was polluted by different levels of polyphenols,polysaccharides or protein.[Conclusion] The genomic DNA extracted by improved CTAB method had a good integrity,high concentration and high purity.The method could be used for DNA experiments with high quality requirements.If follow-up experiments on the DNA quality didn′t ask for much,the kit could be chesen to save time and effort.