Optimization of ethanol extraction process for medicinal materials in Xiahuang Granule prescription by orthogonal test

Objective To optimize the ethanol extraction process for medicinal materials in Xiahuang Granule prescription. Methods Taking the concentration of ethanol, extraction time, amount of ethanol, and extraction times as factors, L 9 (3 4 ) orthogonal test was designed and evaluated with multi-index of yiled and powdered extract yield of five anthraquinones. Results The optimal extraction technology was as follows: reflux extracted twice with 10 times the amount of 60% ethanol, for 1.5 h once. Conclusion This optimized extraction technology is feasible, stable, and reasonable with high yield of active ingredients.