Expression Analysis of Two Genes Related to Male Fertility in A Few Types of Male Sterile Wheat

The various tendency of two target genes' expression in several male sterility wheats was measured to determine whether these genes related to these types of wheat male sterile lines' fertility,and to analyze the key period of abortion in these several male sterility wheats.The total RNA was extracted at various periods of spikelet development at different temperature treatments,then was reverse-transcripted to cDNA with experiment materials,1B/1R K-type wheat male sterile line K3314A and its maintainer line 3314B,non-1B/1R K-type wheat male sterile line 732A and its maintainer line 732B and YS type male sterile line A3017.The expression patterns of 3314B and 732B related to male fertility were analyzed with semi-quantitative PCR.The results showed that there were significant differences in the expression of those two genes among those materials and temperature treatments,except that gene AY914051 in two maintainer lines 3314B and 732B.The expression of the two genes was related to the abortion of pollen development in these male sterile lines with different effects.The expression of the two genes in male sterile lines was affected more significantly by the changing temperature than that in maintainer lines.It can be guessed the key development period of pollen abortion in 1B/1R K-type wheat male sterile line,non-1B/1R K-type wheat male sterile line and YS type male sterile line were the mononuclear period or that between mononuclear period and dinuclear period.The lower temperature might change the expression patterns of genes related to male fertility at different stages,and the accumulation of expression differences likely to eventually lead to the abortion of pollen development.