Establishment of Plant Regeneration System from Medicago sativa L. in Longdong Region

Hypocotyls, cotyledons, leaves and petioles from aseptic seedlings of Medicago sativa L. in Longdong region were used as explants in this study. The basic medium was MS solid medium. The effects of seed coat scratching as well as different growth regulators and their combinations on induction and differentiation of embryogenic callus were observed. The result indicated that seed coat scratching had remarkable influence on seeds germination ratio. The downside of hypocotyl possessed the highest callus induction ratio of 92.2% among all materials. The optimal callus induction medium was MS+2,4-D 2.0 mg·L-1 (same hereinbelow)+NAA 1.0+2.5% sucrose+0.6% agar, the optimal differentiation induction medium was MS+KT 0.5+NAA 0.3+2.5% sucrose+0.6% agar, and the optimal seedling formation medium was 1/2MS+NAA 0.1+1% sucrose +0.6% agar.