Clinical Evaluation of Electro Chemiluminescence Immunoassay for Determination of C-Peptide in Plasma

Objective To evaluate the clinical effectiveness of the detection of Connecting Peptide (C-Peptide) in plasma by electro chemiluminescence immunoassay (ECLIA). Methods Plasma C-Peptide of diabetic patients was detected by ECLIA and enzyme linked immunosorbent assay (ELISA). Insulin was measured by ECLIA simultaneously. Using SPSS software for analysis. Results The coefficient of variations (CVs) of within run and between run of ECLIA was2.13 %, 4.27 %respectively, while that of ELISA was 10.8 % and 16.5 %. The limit of detection of ECLIA was 0.01 ng/mL and ELISA was 0.06ng/mL.The average rate of recovery of ECLIA was 98.9 % and ELISA was 91.9 %. The concentration of plasma c-peptide measured by both ECLIA and ELISA was positively correlated with insulin (r = 0.96, r = 0.89, P 0.05). Conclusion All the technical parameters of ECLIA in measuring C-Peptide are more efficient than those of ELISA. This method is important valuable for clinical application because it is easy and quick to do.