MEASUREMENT OF SERUM INSULIN BY ELECTROCHEMILUMINESCENT IMMUNOASSAY

Objective To evaluate the effectiveness and clinical value of the detection of serum insulin by elctrochemiluminescent immunoassay(ECLIA). Methods Serum insulin of diabetic patients was detected by ECLIA and radioimmunoassay(RIA). C peptide was measured by ELISA simultaneously. Results The coefficient of variations(CVs) of within run and between run of ECLIA was 1.97% and 2.56% respectively,while that of RIA was 10.18% and 13.21%.The average rate of recovery of ECLIA was 99.5% and RIA was 93.4%.The concentration of serum insulin measured by both ECLIA and RIA was positively correlated with c peptide ( r=0.94 , 0.91, P 0.01). Conclusion All the technical parameters of ECLIA in measuring serum insulin gain an advantage over those of RIA. It can be used to judge B cell function of pancreas. This method is valuable for clinical application as it is easy and quick to do.