2005•Biaoji mianyi fenxi yu linchuangRequires access
Evaluation of electrochemiluminescene immunoassay and enzyme-linked immunosorbent assay for serum HBsAb detection
Caiyun Ma, Li Jiang, Ge Gaoxia, Xiaojie Zhang
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Abstract
Electrochemiluminescene immunoassay(ECLIA) and enzyme-linked immunosorbent(ELISA) were used to detect the different concentrations of serum HBsAb, in order to compare the results of ECLIA and ELISA.The result showed that intra-assay coefficient of variation of ECLIA was about 0.95% for high value, 1.13% for mean values and 2.63% for low value, while that of ELISA was about 5.76%, 12.8% and 15.9%, respectively. The interassay coefficient of ECLIA was about 4.03% for high values,4.93% for mean values and 7.34% for low values, while that of ELISA was about 10.1%, 19.6% and 25.2%, respectively.The analytical sensitivity of ECLIA was about 4.0IU/L, while that of ELISA is about 19.0IU/L.Only in 3 samples, the results measured by ECLIA and ELISA were different(ECLIA: positive;ELISA:negative)among 165 samples.It is concluded that the method of ECLIA is more efficient than ELISA for detection of HBsAb in serum.