Influence of Bushen Huoxue Fang on the Expression of Cochlea Fas mRNA in the Kidney-deficiency Rats

Objective:To observe the effect of Bushen Huoxue Fang on the expression of cochlea Fas mRNA in the kidney-deficiency rats.Method: Eight healthy SD rats with a sound auricle response were randomized into the control group,the other 16 rats were modeled into kidney-yang-deficiency by alternate injection of cetacort into their buttocks.After 14 days of modeling,rats were randomized into model group,Bushen Huoxue Fang group.The treatment lasted two weeksn.Rats in each group were chosen randomly to test auditory brain-stem response(ABR) on the day 15 of treatment,then all rats were sacrificed.The blood of the femoral artery was collected to measure the cyclic adenosine monophosphate(cAMP) and cyclic guanosine monophosphate(cGMP).Cochlea was isolated,and RT-PCR method was adopted to test the expressions of Fas mRNA.Result: The Fas mRNA expression in control group was 0.210 1±0.025 6,and 0.551 4±0.090 3 in model group,but Bushen Huoxue Fang was 0.242 9±0.052 5.The model group enhanced the Fas mRNA expression compared with the control group(P0.01);Bushen Huoxue Fang decreased the Fas mRNA expression,compared with model group(P0.01).Conclusion:Bushen Huoxue Fang may regulates the mRNA expression of Fas,therefore can improve the hearing and protect the structure and function of cochlea.