The effect of bushen recipe and huoxue recipe on VEGF in mice of embryo implantation dysfunction

Objective:To observe the effects of bushenantai recipe and its bushen and huoxue components(bushen recipe and huoxue recipe) on the endometrial receptivity in mice of embryo implantation dysfunction. Methods:Using the model of embryo implantation dysfunction induced by indomethacin in mice,to treate with bushenantai recipe,bushen recipe and huoxue recipe.The mice were killed on five and six days of pregnancy,their serum and uterine were collected.To detect the expression of VEGF protein by Radioimmunoassay and to detect the quantity of VEGF mRNA in uterus by RT-PCR. Results:The VEGF protein and mRNA in mice endometrial of bushenantai group were higher than the model group,but the VEGF protein and mRNA of bushen and huoxue group were all a little higher than the model group(except the bushen group of pregnant day 6),but there was no significant difference.Although the comparison of VEGF protein and mRNA of bushenantai group,bushen group,huoxue group were not exactly the same,in generally speaking,the VEGF protein and mRNA of bushen group,huoxue group in the endometrial were less than bushenantai group. Conclusion:Bushenantai recipe can improve the VEGF protein and mRNA levels in endometrial of implantation dysfunction mice,and also can play a role in the promotion of embryo implantation,but using bushen or huoxue recipe alone can not significantly improve the VEGF protein and mRNA levels in mice endometrial.