Research on the impact of Bushen Huoxue Granules on the Wnt/β-catenin signaling pathways in ovariectomized rats

Objective:To explore the mechanism of Bushen Huoxue Granules in preventing and treating osteoporosis by canonical Wnt/β-Catenin signaling pathways.Methods:36 female rats were randomly divided into three groups,model group,sham group,Bushen Huoxue group.The rats in the model group and Bushen Huoxue group were ovariectomized,the rats in the sham group underwent sham treatment,conirming the successful model of osteoporosis.The groups were respectively given 0.9%saline,0.9% saline combined with Bushen Huoxue by intragastric administration every day for 3 months.The rats were killed3 months later.The blood biochemical indicators of estrogen(E2) and indicators of bone formation alkaline phosphatase(ALP),osteocalcin(BGP) were observed by serological testing.BMD and pathology of right femur were observed,and biomechanics of lumbar spine were tested.The expression of β-catenin,Runx2,Osx mRNA of left femur were tested by RT-PCR.Results:Compared with model group,the level of E2in the Bushen Huoxue group was increased(P0.01);bone formation index ALP,BGP increased(P0.05);Bushen Huoxue Granules can signiicantly increase BMD of right femur and mechanical Properties of the lumbar spine(P0.05).The pathological form by HE staining in the Bushen Huoxue group showed that the trabecular bone was slightly smaller,no signiicant thinning,still neatly arranged and connected into a network,density and area were still normal,part of the bone surface area increased slightly.Bushen Huoxue Granules can signiicantly increase the expression of the β-catenin,Runx2,and Osx mRNA of left femur(P0.01).Conclusion:The mechanism of action of Bushen Huoxue Granules prevention and treatment of osteoporosis disease is through the activation of the canonical Wnt/β-Catenin signaling pathway.