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Determination of Chlorogenic Acid in Sargentodoxa cuneata by High Performance Liquid Chromatography

Rong Rong

Open publisher page 1 citations

Abstract

Objective:To establish the determination method of chlorogenic acid in Sargentodoxa cuneata.Method:HPLC was used with Diamonsil ODS-C18 column(4.6 mm×250 mm,5 μm) as chromatographic column and acetonitrile-4% phosphoric acid solution(11∶89) as mobile phase.The flow rate was 1.0 mL · min-1;the detection wavelength was set at 327 nm and the column temperature was kept at 25 ℃.Result:The linear range of chlorogenic acid was 0.000 102-0.010 2 mg.The average recovery was 102.57%,RSD 0.83%(n=6).Conclusion:The method of sample processing was simple and the HPLC method was accurate and reliable.It can be used for the quality control of S.cuneata and its preparation.

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What this paper is about

Objective:To establish the determination method of chlorogenic acid in Sargentodoxa cuneata.Method:HPLC was used with Diamonsil ODS-C18 column(4.6 mm×250 mm,5 μm) as chromatographic column and acetonitrile-4% phosphoric acid solution(11∶89) as mobile phase.The flow rate was 1.0 mL · min-1;the detection wavelength was set at 327 nm and the column temperature was kept at 25 ℃.Result:The linear range of chlorogenic acid was 0.000 102-0.010 2 mg.The average recovery was 102.57%,RSD 0.83%(n=6).Conclusion:The method of sample processing was simple and the HPLC method was accurate and reliable.It can be used for the quality control of S.cuneata and its preparation.

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Available abstract

Objective:To establish the determination method of chlorogenic acid in Sargentodoxa cuneata.Method:HPLC was used with Diamonsil ODS-C18 column(4.6 mm×250 mm,5 μm) as chromatographic column and acetonitrile-4% phosphoric acid solution(11∶89) as mobile phase.The flow rate was 1.0 mL · min-1;the detection wavelength was set at 327 nm and the column temperature was kept at 25 ℃.Result:The linear range of chlorogenic acid was 0.000 102-0.010 2 mg.The average recovery was 102.57%,RSD 0.83%(n=6).Conclusion:The method of sample processing was simple and the HPLC method was accurate and reliable.It can be used for the quality control of S.cuneata and its preparation.

Key concepts: Chlorogenic acid, Chromatography, Phosphoric acid, High-performance liquid chromatography, Chemistry, Acetonitrile, Linear range, Detection limit

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