Studies on Calli Liquid Culture and Plant Regeneration of Konjac

The granular calli of konjac was employed for study of liquid culture and plant regeneration.The results showed that MS+BA 1.0 mg/L+2,4-D 0.2 mg/L+ sugar 30 g/L+ agar 6.0 g/L could efficiently induce granular calli from the bud.Obtained calli were cut about 0.3 cm and then subsequently cultured in MS+BA 0.05 mg/L+NAA 0.1 mg/L+sugar 30 g/L+PVP(PolyVinyl-Pyrrolidone)1.0 g/L for establishing liquid culture system.The growth of liquid culture material showed a parabola curve,the weight gain for more than 80% of entire culture period from the sixth day to the twelfth day.In addition,pH value of medium increased during the culture process.The material that was cultured twelve days turned to 1/2MS+BA 0.6 mg/L +NAA 0.1 mg/L+glucose 30 g/L +vitamin C100 mg/L+agar 6.0 g/L for differentiation culture.Big buds that the leaf expects were cut and put on 1/2MS+ +NAA 0.1 mg/L +sugar 30 g/L+agar 6.0 g/L for inducing root,eventually the regenerative plantlets were formed.