2011•Shandong Agricultural SciencesRequires access
Tissue Culture of Chinese Cherry Cultivar Taixiaohongying
Xiaoliu Chen
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Abstract
The shoots of Chinese cherry cultivar Taixiaohongying were used as explants and cultured on WPM and MS media with different concentrations of 6-BA and NAA.The culture effects were studied and the results were as follows.(1) The best culture medium for shoots was MS+1.0 mg/L 6-BA+0.1 mg/L NAA.On this medium,the axillary buds sprouted easily and grew vigorously.(2) The best multiplication culture medium was WPM+1.0 mg/L 6-BA+0.1 mg/L NAA.On this medium,the multiplication of axillary buds and cluster buds coexisted,so the regeneration multiple increased significantly and reached eight times;the regenerated shoots grew vigorously and had dark green leaves.(3) The best rooting medium was 1/2MS+0.5 mg/L IBA+20 g/L sucrose+5 g/L agar.On this medium,the rooting rate was higher,and the roots were longer and more.